Bring the power of Absolute Q® to your research
Digital PCR can empower a variety of applications. Find yours and see what the Absolute Q can do for you!
- Absolute Quantification
- Gene Editing
- Infectious Disease
- Medium Multiplexity
- Reproductive Health
- Wastewater Monitoring
In this study, we demonstrate the performance of a novel 4-color multiplexing non-invasive prenatal trisomy test on the Absolute Q dPCR platform to simultaneously detect T13, T18 and T21.
Emulsion-free Digital PCR Measurement of Wastewater Related Targets using the SARS-CoV-2 Wastewater Surveillance 4-plex Assay
Wastewater based epidemiology (WBE) enables tracking of biomarkers for specific pathogens to monitor for disease outbreak and spread. WBE’s utility in disease surveillance has been proven to be effective in monitoring for rare cases of disease.
Screening wastewater, or sewage, for the presence of SARS-CoV-2 viral RNA can be an effective orthogonal monitoring method in addition to ongoing clinical testing. Wastewater serves as pooled samples from members of a community and enables broad collection of surveillance data.
RNA extraction is a critical step in COVID-19 molecular testing. The loss of viral RNA during the extraction step can result in false negatives. Therefore, optimization of the RNA extraction protocol to ensure consistent and high yield recovery of viral RNA could potentially improve COVID-19 testing accuracy.
In this application note created in collaboration with Integrated DNA Technologies, we demonstrate 4-color optical multiplexing for multi-allele single nucleotide polymorphism discrimination.
CRISPR-Cas 9 technology has been rapidly adopted due to its ability to make precise edits in the genome. Here, we demonstrate using the Absolute Q to accurately identify both knock-ins and knock-outs.
In this study we tested nucleic acids extracted from 19 clinical specimens using the Absolute Q to demonstrate the improved sensitivity and accuracy of RT-dPCR over traditional RT-qPCR.
Leveraging the Absolute Q’s qPCR-like workflow, we showcase the multiplexed 1-step |Q| SARS-CoV-2 RT-dPCR assay by quantifying viral target input with high accuracy down to 5 copies per reaction.
Monitoring of the BCR-ABL1 fusion gene is integral to successful TKI treatment in Chronic Myeloid Leukemia. See how the Absolute Q provides accurate measurement of the BCR-ABL1/ABL1 ratio.
Spinal muscular atrophy (SMA) is a leading genetic cause of infant mortality. Here, we describe a 4-plex digital PCR assay capable of quantifying the copy numbers of SMN1 and SMN2 genes, which are central to SMA.