RNA extraction is a critical step in COVID-19 molecular testing. Loss of viral RNA during the extraction step can result in false negatives. Therefore, optimization of the RNA extraction protocol to ensure consistent and high yield recovery of viral RNA could potentially improve COVID-19 testing accuracy. The goal of this study is to demonstrate how digital PCR can be used to optimize conditions for viral RNA extraction using verified molecular controls. Digital PCR may also be used as a quality control tool to monitor sample preparation consistency across facilities and labs.
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