Absolute quantification without the standard curve

Digital PCR (dPCR) partitions a bulk PCR reaction into thousands of picoliter-scale reactions — each containing one or just a few DNA molecules. By simply counting positive partitions, the original targets can be precisely quantified without needing a standard curve.

Digital PCR provides unparalleled confidence in applications ranging from rare target identification, copy-number-variation (CNV), gene expression and more.

Highlighted Research Areas

Oncology

From routine biomarker testing to long-term patient monitoring, digital PCR (dPCR) with the Absolute Q System enables high confidence in target identification — down to just a few target molecules per reaction. Learn how Absolute Q leverages low false positives and multi-color multiplexing to enhance precision medicine with a one-step, walk-away workflow.

Infectious Disease​​

Digital PCR is ideal for identifying rare sequences that are lost in the background as is often the case for infectious disease analysis. Furthermore, Digital PCR reliably outperforms qPCR in ultra-rare target detection and is more tolerant to potential PCR inhibitors. Learn more about how the Absolute Q’s 4-color multiplexing and 90-minute workflow can be leveraged for infectious disease applications.

Reproductive Health​​

Digital PCR is ideal for identifying rare sequences that are lost in the background as is often the case for infectious disease analysis. Furthermore, Digital PCR reliably outperforms qPCR in ultra-rare target detection and is more tolerant to potential PCR inhibitors. Learn more about how the Absolute Q’s 4-color multiplexing and 90-minute workflow can be leveraged for infectious disease applications.

Highlighted Applications

  • All
  • Gene Editing
  • Infectious Disease
  • Oncology
  • Reproductive Health
All
  • All
  • Gene Editing
  • Infectious Disease
  • Oncology
  • Reproductive Health

4-Color Discrimination of Multi-Allele Single Nucleotide Polymorphisms on the Absolute Q

In this application note created in collaboration with Integrated DNA Technologies, we demonstrate 4-color optical multiplexing for multi-allele single nucleotide polymorphism discrimination.

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Evaluation of CRISPR-Cas9 Mediated Genome Edits with Absolute Q Digital PCR platform

CRISPR-Cas 9 technology has been rapidly adopted due to its ability to make precise edits in the genome. Here we demonstrate using the Absolute Q to accurately identify both knock-ins and knock-outs.

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Testing qPCR positive, negative and inconclusive COVID-19 clinical samples using digital PCR

In this study we tested nucleic acids extracted from 19 clinical specimens using the Absolute Q to demonstrate the improved sensitivity and accuracy of RT-dPCR over traditional RT-qPCR.

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|Q| SARS-CoV-2 Triplex Assay: Multiplexed 1-step RT-dPCR for accurate viral target detection

Leveraging the Absolute Q’s qPCR-like workflow, we showcase the multiplexed 1-step |Q| SARS-CoV-2 RT-dPCR assay by quantifying viral target input with high accuracy down to 5 copies per reaction.

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Discriminatory 11-Plex Assay Designed to Monitor Estrogen Receptor Mutations Using a Novel, Melt-Analysis Capable Digital PCR Platform​

Luminex has developed an 11-plex assay on the Absolute Q to detect SNPs in the ESR1 gene. This poster details the study that confirms the assay’s ability to detect down to 0.1% of minor allele fraction.

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Fully Integrated Single Instrument Imaging-based Digital PCR Platform

The Combinati Absolute Q is the only digital PCR platform that integrates walk-away workflow, fast time to result, AI-power image processing, and unparalleled partitioning consistency.

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