|Q| SARS-CoV-2 Triplex Assay

Accurate, precise and sensitive coronavirus detection for COVID-19 research using digital PCR

Overview

Designed for use on the Absolute Q Digital PCR platform, the |Q| SARS-CoV-2 Triplex Assay provides unmatched low-end sensitivity and a streamlined multiplex assay using a single instrument with an easy workflow. This multiplex assay is designed for the quantitative detection of nucleic acid from the SARS-CoV-2 in respiratory specimens and sera in a single reaction step, and is based on the CDC COVID-19 EUA assays.  For Research Use Only.

Product Highlights

Sensitive 

Detect down to just a few copies of viral RNA

 

Fast

Get testing results in under 80 minutes

Familiar

qPCR-like testing workflow is easy to set up and scale

Efficient

1-tube quantification of 3 assay targets with 4-color multiplexing capabilities

Downloads

1-Step RT-dPCR in under 80 minutes on the Combinati Absolute Q

Tech Note

Reverse Transcription PCR (RT-PCR) is an important tool that allows the assessment of nucleic acid targets that are present in the form of RNA. It has a wide range of applications including gene expression and detection of RNA viruses.

DOWNLOAD

Combinati |Q| SARS-CoV-2 Triplex Assay for sensitive detection of viral RNA targets​

App Note

The COVID-19 pandemic has drawn heightened concern, with over eleven million positive SARS-CoV-2 cases confirmed worldwide by July 2020. 1 RT-qPCR currently serves as the clinical standard for the diagnosis of COVID-19.

DOWNLOAD the App Note

Combinati |Q| SARS-CoV-2 Digital PCR Test Protocol​

Protocol

This document describes the use of the SARS-CoV-2 Digital PCR (dPCR) Kit on COMBiNATi Absolute Q digital PCR instrument. The kit contains the 2019-nCoV CDC dPCR Triplex Probe Assay and 1-Step RT dPCR Supermix. 

DOWNLOAD

Absolute quantification of coronavirus transcripts without a standard curve

With a limit of detection of 5 copies of viral RNA, Combinati’s |Q| SARS-CoV-2 Triplex Assay achieves unmatched sensitivity for identifying low-abundance viral transcripts.

Dynamic range of SARS-CoV-2 quantification on Absolute Q using |Q| SARS-CoV-2 Triplex Probe Assay
Dynamic range testing for 5, 100, an 1000 SARS-CoV-2 RNA targets among 50 nanograms (~15,000 copies) of human genomic DNA. Individual points are plotted.

Increase testing efficiency with 4-color multiplexing

Absolute Q overcomes the multiplexing limitations seen with other dPCR platforms, enabling streamlined testing of up to four targets in a single well. The |Q| SARS-CoV-2 Triplex Assay targets the N1 and N2 gene sequences as well as the human RnaseP gene.

Simplex vs multiplex testing of the |Q| SARS-CoV-2 Triplex Assay components. All reactions were performed using control material containing all three targets of interest. Simplex assays (N1, N2, and RNAse P) detect only one target per reaction, whereas the triplex assay detects all three in a single reaction.

Best-in-class data consistency

Reproducibility testing of the Combinati |Q| SARS-CoV-2 Triplex Assay shows high levels of concordance between runs and replicates. Duplicate reactions of positive and negative were run in duplicate for two control material across four instruments for a total of eight replicates each.

A comparison of results across sites using a reference standard demonstrates extremely high levels of reproducibility, making this platform ideal for both longitudinal studies as well as multisite collaborations. 

Sample to answer in just 80 minutes

With a dramatically simpler workflow than other digital PCR platforms, Absolute Q requires no droplet preparation or plate scanning. Simply prepare your reagents the way you would set up a qPCR assay, load the reagents onto a MAP16 plate, and start the instrument. That’s it.

Prepare

Reagent preparation is identical to qPCR

Load

dPCR reagents are loaded onto MAP16 plate and gaskets applied

Start

Sample partitioning, reverse
transcription, thermal cycling,
and dPCR data acquisition
one instrument – 80 minutes

Prepare

Reagent preparation is identical to qPCR

Load

dPCR reagents are loaded onto
MAP16 plate and gaskets applied

Start

Sample partitioning, reverse
transcription, thermal cycling,
and dPCR data aquisition
one instrument – 90 minutes

Contact Us