How does Digital PCR help you?

Digital PCR (dPCR) partitions a bulk PCR reaction into thousands of picoliter-scale reactions — each containing one or just a few DNA molecules. By simply counting positive partitions, the original targets can be precisely quantified without needing a standard curve.

Digital PCR provides unparalleled confidence in applications ranging from rare target identification, copy-number-variation (CNV), gene expression and more.

Highlighted Research Areas

Infectious Disease

Digital PCR is ideal for identifying rare sequences that are lost in the background as is often the case for infectious disease analysis. Furthermore, Digital PCR reliably outperforms qPCR in ultra-rare target detection and is more tolerant to potential PCR inhibitors. Learn more about how the Absolute Q’s 4-color multiplexing and 90-minute workflow can be leveraged for infectious disease applications.
COVID-19

Oncology

From routine biomarker testing to long-term patient monitoring, digital PCR (dPCR) with the Absolute Q System enables high confidence in target identification — down to just a few target molecules per reaction. Learn how Absolute Q leverages low false positives and multi-color multiplexing to enhance precision medicine with a one-step, walk-away workflow.
Download: Precision cancer monitoring using  a digital PCR Platform

Reproductive Health

A core consideration of reproductive health is empowering patients to make the best health decisions. With its unparalleled accuracy and precision, digital PCR provides patients the information they need. From genetic disease screening to residual disease monitoring, the Absolute Q has the potential to bring dPCR closer to patients, enabling more timely decisions.
 Download: SMA app note

Highlighted Applications

  • All
  • Absolute Quantification
  • Copy Number Variation
  • Gene Expression
  • Genotyping
  • Rare Target Detection
All
  • All
  • Absolute Quantification
  • Copy Number Variation
  • Gene Expression
  • Genotyping
  • Rare Target Detection

SARS-CoV-2 Reference Material Quantification on the Absolute Q dPCR Platform

To address the need for a reliable method of accurate and precise measurements of SARS-CoV-2 samples, we leverage the Combinati Absolute Q Digital PCR (dPCR) platform, to quantify commercially available SARS-CoV-2 reference control material.

APPLICATION NOTE

IDT rhAmp EGFR p.T790M Genotyping on the Combinati Absolute Q

Integrated DNA Technologies’ rhAmp SNP Genotyping System enables highly precise interrogation of SNPs within difficult genomic regions. For the first time, the IDT rhAmp assay performance was demonstrated on a digital PCR platform — quantifying EGFR p.T790M mutation down to 0.1%.

APPLICATION NOTE

BCR/ABL Gene Expression Assay on the Combinati Absolute Q

95% of Chronic Myeloid Leukemia (CML) cases are characterized by the presence of a BCR-ABL1 fusion gene. Regular monitoring involving precise quantification of BCR-ABL1 transcripts is integral to successful tyrosine kinase inhibitor (TKI) treatment. Read more about how samples with precise ratios of BCR-ABL1/ABL1 were created and confirmed on the Combinati dPCR platform.

APPLICATION NOTE

Quadruplex Absolute Q Digital PCR for Spinal Muscular Atrophy (SMA) Screening

Spinal muscular atrophy (SMA), a genetic neuromuscular disorder and leading genetic cause of infant mortality. Here we describe a 4-color multiplexing digital PCR solution on the Combinati Absolute Q to meet the growing demand for rapid SMA newborn screening and treatment.

APPLICATION NOTE

TaqMan™ Liquid Biopsy assays for rare target quantification

Digital PCR (dPCR) enables rare target detection even in the presence of high non-target background. Read more about how the Applied Biosystems™ TaqMan™ Liquid Biopsy dPCR Assays were used to perform rare target detection for 5 hot-spot cancer mutations on the Combinati Absolute Q platform.

APPLICATION NOTE