Determining the Effects of Fragment Length on Digital PCR Dynamic Range and Optimizing Library Quantification

In order to maximize Illumina next generation sequencing (NGS) runs, the loading concentration of NGS libraries must be carefully controlled. Both over- and under-loading will decrease the amount of usable reads by limiting the number of sequenceable clusters. Library quantification using digital PCR (dPCR) is commonly used for accurate concentration determinations of complete and sequenceable library fragments. Download the poster to read more about a study conducted between the Combinati Absolute Q®  and the BioRad QX200 to perform NGS library quantification across libraries with a range of fragment lengths. 

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