Combinati’s Absolute Q® is the only digital PCR system with unparalleled accuracy and a workflow identical to qPCR.
Combinati’s Absolute Q® is the only digital PCR system with unparalleled accuracy and a workflow identical to qPCR.
Designed for use on the Absolute Q Digital PCR platform, the SARS-CoV-2 Wastewater Surveillance 4-plex Kit is formulated specifically for monitoring SARS-CoV-2 in wastewater. Leveraging the 4-color multiplexing capability of the Absolute Q, the assay quantifies the N1 and N2 gene from SARS-CoV-2 along with Pepper Mild Mottle Virus (PMMoV) for a human fecal control and Bovine Coronavirus (BCoV) as an integrated process control target all in a single reaction.
Designed for use on the Absolute Q Digital PCR platform, the SARS-CoV-2 Wastewater Surveillance 4-plex Kit is formulated specifically for monitoring SARS-CoV-2 in wastewater. Leveraging the 4-color multiplexing capability of the Absolute Q, the assay quantifies the N1 and N2 gene from SARS-CoV-2 along with Pepper Mild Mottle Virus (PMMoV) for a human fecal control and Bovine Coronavirus (BCoV) as an integrated process control target all in a single reaction.
Quantify down to just a few copies of viral RNA
Get results in under 2 hours
qPCR-like workflow that’s easy to set up and scale
1-tube quantification of 3 assay targets
1-tube quantification of 3 assay targets
Workflow is similar to
qPCR and easy to set
up and scale
Get results in under 2 hours
Detect down to just a
few copies of viral RNA
Tracking SARS-CoV-2 levels in wastewater has been shown to be a useful predictor of potential outbreaks.1 However, for meaningful interpretation of SARS-Cov2 data, a normalization target is critical. The SARS-CoV-2 Wastewater Surveillance 4-plex Kit utilizes the Pepper Mild Mottle Virus (PMMoV) as a human fecal load control as recommended by the CDC.
In this Application Note, the Absolute Q and SARS-CoV-2 Wastewater Surveillance 4-plex Kit were used to test wastewater samples collected for an University on-campus monitoring effort.
Tracking SARS-CoV-2 levels in wastewater has been shown to be a useful predictor of potential outbreaks.1 However, for meaningful interpretation of SARS-Cov2 data, a normalization target is critical. The SARS-CoV-2 Wastewater Surveillance 4-plex Kit utilizes the Pepper Mild Mottle Virus (PMMoV) as a human fecal load control as recommended by the CDC.
The SARS-CoV-2 Wastewater Surveillance 4-plex Assay also includes a process control target (BCoV) to enable precise quantification of nucleic acid recovery during sample processing.
By spiking in a quantified control material, the Absolute Q’s accurate output allows precise measurement of extraction efficiency across different samples.
The SARS-CoV-2 Wastewater Surveillance 4-plex Assay also includes a process control target (BCoV) to enable precise quantification of nucleic acid recovery during sample processing.
By spiking in a quantified control material, the Absolute Q’s accurate output allows precise measurement of extraction efficiency across different samples.
Our application scientists can walk you through how to get started.
1. Betancourt WW, et al., Wastewater-based Epidemiology for Averting COVID-19 Outbreaks on The University of Arizona Campus. medRxiv. 2020.11.13.20231340.
