Digital PCR (dPCR) is quickly becoming the new gold standard in precise nucleic acid quantitation. In addition to higher sensitivity and more robust performance in the face of inhibitors, the Absolute Q leverages unique partitioning technology to minimize reagent waste and maximize the accuracy of your dPCR results. Learn how dPCR is being leveraged in a number of research applications to provide unparalleled confidence in rare target quantification, copy-number-variation (CNV), gene expression, and more.
Discover all the ways digital PCR can help tackle your research challenges
Wastewater and Environmental Health
Screening wastewater, or sewage, for the presence of SARS-CoV-2 viral RNA is gaining steam as an effective method of community monitoring for COVID-19. Learn how digital PCR provides unparalleled sensitivity and high confidence in target detection from challenging sample input types.
Rare Target Detection
For many molecular diagnostic applications such as detection of circulating tumor DNA in liquid biopsy, accuracy and precision are required due to the relatively low target abundance. Precise measurement of rare nucleic acid is most accurate when more of the original sample is analyzed. Learn more about how the Absolute Q’s high loaded sample volume is being applied for more accurate rare target detection.
Copy Number Variation
Copy number variations represent a large portion of natural variability of the human genome. Digital PCR, which utilizes simple counting of target molecules to achieve absolute quantification, enables higher precision and accuracy to detect and quantify copy number variations – even if the changes are small. Absolute Q provides four optical channels for multiplexing, enabling more genes to be interrogated per reaction – requiring fewer samples when compared to qPCR.
Accurate quantification is important for a variety of applications such as determining CRISPR editing efficiency, the concentration of next generation sequencing libraries, or detecting small variances in gene expression. Coupling a workflow that’s designed to be identical to qPCR with 4-color multiplexing capability, Absolute Q allows users to easily transition current qPCR assays to dPCR with minimal modifications – saving time and improving data quality.