Infectious Disease Research using Digital PCR

Infectious Disease Research using Digital PCR

infectious-disease-dpcr

Digital PCR delivers more accurate results with a wider dynamic range

Digital PCR (dPCR) works by partitioning a bulk PCR reaction into thousands of nanoliter-scale reactions — each containing zero, one, or just a few DNA molecules.

Recently, RT-dPCR (reverse transcription digital PCR) has been shown to outperform traditional RT-qPCR in terms of sensitivity and accuracy in identifying patients who are infected with the SARS-CoV-2 virus. The Absolute Q digital PCR platform combines a streamlined workflow, consistent hands-off partitioning, and 4-color multiplexing to offer the most accurate digital PCR solution for viral load quantification.

Webinar: Multiplex 1-Step RT-dPCR for SARS-CoV-2

Learn more about how the Absolute Q can accurately quantify as few as 5 viral targets per reaction using the 1-step |Q| SARS-CoV-2 Triplex Assay kit.

Sensitive detection of viral RNA targets

In collaboration with USC Clinical Laboratories, Molecular Pathology at University of Southern California, we tested nucleic acids extracted from 19 clinical specimens using the Absolute Q to demonstrate the improved sensitivity and accuracy of RT-dPCR over traditional RT-qPCR.

1-Step RT-dPCR in under 90 minutes

In this technical note, we showcase the simple workflow of reverse-transcription digital PCR (RT-dPCR) on the Absolute Q. Using the |Q| SARS-CoV-2 Triplex Assay, accurate quantitation of SARS-CoV-2 viral targets was completed in under 2 hours with a single hands-on step.

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