Discover the technical capabilities of the Absolute Q®

Do you have a complex question that you think digital PCR could answer? Learn more about the Absolute Q’s unique design and microfluidic array partitioning technology to discover how digital PCR can enhance your research efforts.

Absolute Q Digital PCR Platform Technical Notes

Robust Quantification of Next Generation Sequencing Libraries using Absolute Q Digital PCR

Advances in next-generation sequencing (NGS) technologies had accelerated the discovery of actionable genomic targets. Accurate quantification of the final library products is critical to maximizing both data quality and output. Multiple studies have demonstrated that utilizing digital PCR (dPCR) as a quantification tool for NGS libraries before sequencing helps optimize sequencing run performance, data generation, and data quality.

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Improve sensitivity for rare targets by increasing total analyzed sample using digital pooling of digital PCR data

For samples with very rare targets, the volume of sample input may impact the ability to detect ultra-rare events. In this technical note, the Digital Pooling feature of the Absolute Q Analysis Software is highlighted by showcasing rare target detection of mutation allele frequencies (MAF) at 0.1% using multiple MAP16 dPCR arrays.

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Using in-line process controls to evaluate sample processing workflow efficiency

An in-line process control is intended to provide quantitative information about sample loss during sample processing workflow. Typically, a process control is spiked into the initial sample before any processing takes place.

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Digital PCR Wastewater Surveillance: Detect SARS-CoV-2 alongside human fecal and process controls

In this study, we characterize the LoD of the Combinati |Q| SARS-CoV-2 RT-dPCR Triplex Kit by diluting reference materials into a pooled negative matrix to determine the lowest concentration at which the assay can reliably identify the sample as containing SARS-CoV-2 targets.

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Establishing the Limit of Detection for the |Q| SARS-CoV-2 Triplex Assay

In this study, we characterize the LoD of the Combinati |Q| SARS-CoV-2 RT-dPCR Triplex Kit by diluting reference materials into a pooled negative matrix to determine the lowest concentration at which the assay can reliably identify the sample as containing SARS-CoV-2 targets.

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1-Step Reverse Transcription Digital PCR (RT-dPCR) in Under 2 Hours

Reverse transcription allows the detection and quantification of RNA. Here, we demonstrate a true 1-step RT-dPCR workflow on the Absolute Q by quantifying SARS-CoV-2 targets in under 90 minutes.

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